Title : In vitro therapeutic trial on the effect of curcuma L and vit C on Enterococcus faecalis and Candida albicans in recurrent endodontic infections
Abstract:
Background: The success of endodontic treatment relies heavily on effective root canal irrigation. This crucial step helps prevent bacterial resistance from pathogens like Enterococcus faecalis and Candida albicans, which are often responsible for recurrent infections.
Aim: To evaluate the effectiveness of both stabilized and non-stabilized vitamine C and Curcuma longa L. essential oil against Enterococcus faecalis and three strains of Candida albicans, which are associated with recurrent endodontic infections.
Methods: The antimicrobial activity was assessed against four microbial strains using the well diffusion method for qualitative evaluation and the microplate method to determine the minimum inhibitory concentration (MIC).
Results: The study revealed that essential oil solutions with ethanol showed the lowest MIC values compared to those with Tween 80 and distilled water, particularly against Enterococcus faecalis. Stabilized vitamin C demonstrated significantly lower MIC values than unstabilized vitamin C, especially against E. faecalis and Candida albicans ATCC 10231, highlighting its superior antimicrobial activity. Similar MIC values were observed for both forms of vitamin C against Candida albicans ATCC 26790 and IP444. These results emphasize the enhanced efficacy of stabilized vitamin C and ethanol-based solutions.
Conclusion: Stabilized vitamin C showed significantly lower MICs than its unstabilized form, particularly against E. faecalis. Additionally, Curcuma longa L. essential oil exhibited notable antimicrobial activity, with ethanol-based solutions being the most effective.
Keywords: Recurrent endodontic infection, Enterococcus faecalis, Candida albicans, vitamin C, essential oil of Curcuma longa L.
