HYBRID EVENT: You can participate in person at Rome, Italy or Virtually from your home or work.
September 16-18, 2024 | Rome, Italy
FAT 2023

Sinead Ryan

Sinead Ryan, Speaker at Food Science Congress
University College Dublin, Ireland
Title : Improving the oral delivery of food derived peptides with antihypertensive properties


Isoleucine-Proline-Proline (IPP) and Leucine-Lysine-Proline (LKP) are food-derived tripeptides whose antihypertensive functions have been demonstrated in hypertensive rat models. However, peptides display low oral bioavailability due to poor intestinal epithelial permeability and instability. IPP and LKP were formulated into nanoparticles using chitosan via ionotropic gelation and then coated with zein. Following addition of zein, a high encapsulation efficiency of 80% was obtained for the nanoparticle. In simulated gastric fluid, 20% cumulative release of the peptides was achieved after 2 h, whereas in simulated intestinal fluid, ~90% cumulative release was observed after 6 h. Higher colloidal stability (39-41 mV) was observed for the coated NP compared to uncoated ones (30-35 mV). In vitro cytotoxicity studies showed no reduction in cellular viability of human intestinal epithelial Caco-2 and HepG2 liver cells upon exposure to nanoparticle and nanoparticle components. Administration of nanoparticle encapsulating IPP and LKP by oral gavage to spontaneously hypertensive rats attenuated systolic blood pressure for 8 h. This suggests that the nanoparticle provide appropriate release to achieve prolonged hypotensive effects in vivo. In conclusion, chitosan-zein nanoparticles have potential as oral delivery system for the encapsulation of IPP and LKP.

What will audience learn from your presentation?

  • Convert low value industrial co-streams into high value food supplements.
  • Food derived peptides with a health promoting effect on lifestyle diseases such as hypertension.
  • In this research we have used pharmaceutical drug assessment methods (in vitro, ex-vivo and in vivo) and adapted them for nutraceutical/food bioactive assessment.


Dr. Sinead Ryan completed her PhD in the Alimentary Pharmabiotic Centre, University College Cork, Ireland. Later she completed a Postdoctoral Research Fellow in University College Dublin, Ireland where she moved to a new discipline – research area of drug delivery. Her research focused on the oral delivery of novel mucoadhesive polymeric peptide conjugates. She later joined The Irish Drug Delivery Research Network as Senior Postdoctoral Research Fellow where she gained expertise in overcoming barriers for efficient delivery of peptides for oral, injectable, and intra-articular delivery for osteoarthritis. Currently, her research area is multidisciplinary, involving the design and assessment of formulations for peptide and food derived peptide (nutraceutical) oral delivery. Her current position is Assistant Professor in the School of Veterinary Medicine in University College Dublin.