Antioxidant activity and protective effect of Lindera glauca Blume stem extracts on t-BHP-induced oxidative stress in vitro and in vivo

Title : Antioxidant activity and protective effect of Lindera glauca Blume stem extracts on t-BHP-induced oxidative stress in vitro and in vivo

Abstract:

I n this study, we investigated the antioxidant and protective effect of Lindera glauca (L. Glauca) stem (LGS) extracts. We compared antioxidant properties of water extract (LGSW) with ethanol extract (LGSE) by determining the contents responsible for antioxidant activities such as polyphenols and flavonoids. Antioxidant properties were also determined by ferric reducing antioxidant power (FRAP), and 2,2’-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical scavenging activity. Both LGSW and LGSE showed a strong inhibitory effect on lipid peroxidation measured by ferric thiocyanate (FTC) and thiobarbituric acid (TBA) values. Especially, LGSE showed a protective effect by increasing cell viability, decreasing intracellular reactive oxygen species (ROS) against tert-butyl hydroperoxide (t-BHP)-induced oxidative stress in Chang cells. Furthermore, LGSE showed the increased antioxidant related enzyme such as catalase, GST, GPX, and SOD gene expression against oxidative stress in zebrafish model. Our findings suggest that LGSE could be useful to develop potential therapeutic agents with protective antioxidant effects against oxidative stress.

Biography:

Dr. Yon-Suk Kim is working as postdoctoral fellow in the Department of Biotechnology, Konkuk University, Chungju, South Korea. Her current area of research is to elucidate the role of bioactive compounds found in bio-resources. She received her Ph.D. degree from Konkuk University, South Korea, in understanding the importance of antioxidant and anti-infammatory effects of plant extracts and their bioactive compounds. She has published more than 51 research papers in SCI journals.