Title : Green synthesis, biochemical characterization and antibacterial activity from Dioscorea pentaphylla
Abstract:
Ethnomedicinal research offers valuable insights for the advancement of drug discovery through the utilization of medicinal plants, which are essential natural resources crucial for human well-being. Medicinal plants represent as an alternative treatment to various diseases, and their use is increasingly prevalent throughout the world. The highly medicinal dioecious Dioscorea of Dioscoreaceae contains more than 600 species globally. The Dioscorea rhizomes are renowned for their ethnobotanical, Nutritive, antioxidant, and biological potentiality that ensure the quality of daily nourishment of the indigenous people. Nanotechnology encompasses a diverse field of study that explores the various characteristics of nanoparticles. in nutrients and herbal medicines, The Qualitative screening for phytochemicals revealed the presence of both flavonoids and many other phenolic components have been reported for their effective antioxidants, anticancer, antibacteria, cardioprotective agents, anti-inflammation, immune system promoting, skin protection from UV radiation, and interesting candidate for pharmaceutical and medical application. The GCMS analysis of the ethanolic extract of Dioscorea pentaphylla rhizome showed the presence of 20 phytocompounds.The bio reduction initiated which revealed the UV spectral peak sharp and stable peak at 250 - 475 nm which clearly emphasizes the Nano synthesis has been initiated in the Dioscorea pentaphylla rhizome extract by the metal silver nitrite. FTIR analysis reveals the Dioscorea pentaphylla rhizome extracts possess a wide range of functional groups namely carboxylic acids, alkanes, alcohols, organic acids , aromatic compound and halogen respectively. The Scanning Electron Micrograph (SEM) of the present analysis clearly picturized the oval, cuboidal and spherical structural morphology which may be due to the optical groups aiding in the stability of the above structures (spherical/oval). The amount of total polysaccarides. ,protein content amount of protein (3.5 ±7.8). The cholesterol (2.5 ± 0.01) levels in Dioscorea pentaphylla rhizome was notified to be significant. The mineral calcium, (14.5 ± 27.4) and antioxidant vitamins A and vitamin C. The Antibacterial study provided an effective inhibitory activity against the selected strains (E.coli and Bacillus subtilis). Invitro antioxidant status of Dioscorea pentaphylla rhizome extract exhibited notable antioxidant radical scavenging activity, DPPH showed effective antioxidant potential.
