Title : Determination of antibiotic residues in milk marketed in Saudi Arabia
Abstract:
Antibiotic resistance has emerged as a major global health issue since it is thought to be the cause of approximately 0.7 million annual deaths, with that number predicted to increase to 10 million by 2050. On other hand, Residues of antibiotics detected in animal-derived food items stem from the application of veterinary medications, showcasing the likelihood of these compounds transferring into edible tissues throughout the animal’s life cycle. Antibiotics are predominantly found as residues in milk due to their utilization in treating animal infectious diseases. Additionally, improper use of certain antibiotics as feed additives can contribute to antibiotic residues in milk. The objective of this study is to identify and detection the levels of antibiotics in milk samples obtained from Saudi markets, involving the collection of 20 samples, using solid phase extraction method, followed by analysis through liquid chromatography-tandem mass spectrometry (LC-MS/MS). In the LC-MS/MS method, the recovery value ranges from 70 to 120, the limit of quantification (LOQ) was ranged from 3.40 ug/kg to 24.35 µg/kg which was calculated based on the slope of the calibration curve of different analytes. Fortunately, all analyzed samples were below LOQ except sulfamethazine which was detected in one of samples with concentration 16.784 ug/kg. In conclusion, the Saudi FDA plays a crucial role in overseeing and regulating antibiotics in milk to safeguard public health, with a focus on controlling the use of approved drugs in lactating animals.
